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. Author manuscript; available in PMC: 2007 Nov 1.
Published in final edited form as: Free Radic Biol Med. 2007 Apr 10;43(1):117–127. doi: 10.1016/j.freeradbiomed.2007.04.005

Fig. 4.

Fig. 4

Effect of cyanide on BNIP3, BAX and Bcl-2 gene expression. (A) The mRNA levels of BNIP3, BAX and Bcl-2 in cells treated with 400 μM KCN as determined by real-time PCR. Levels of mRNA are presented relative to β-actin. (B) BNIP3, BAX and Bcl-2 expression was determined by Western blotting at different durations of 400 μM KCN exposure. (C) Cells were treated with different concentrations of KCN or transfected with BNIP3 cDNA for 24 h, followed by Western blotting of BNIP3. Data represent mean ± SEM of 3 separate determinations. *Significantly different from control (without KCN treatment) group, P<0.05.

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