Fig. 5.

HIF-1α silencing reduced BNIP3 expression in cyanide treated cells. (A) Cells were transfected with either control siRNA or siRNA specific for HIF-1α for 36 h, then treated with 400 μM KCN for 3 h. The HIF-1α level was determined by Western blotting. (B) HIF-1α siRNA transfected cells were treated with 400 μM KCN for 9 h, then BNIP3 mRNA levels were determined by real-time RCR. (C) Cells were transfected as in (B), then exposed to 400 μM KCN for 24 h. The level of BNIP3 expression was determined by Western blotting. Data represent mean ± SEM of 3 separate determinations. *Significantly different from control RNAi treatment group; #signficantly different from the KCN + RNAi control group. P<0.05.