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. Author manuscript; available in PMC: 2007 Nov 1.
Published in final edited form as: Free Radic Biol Med. 2007 Apr 10;43(1):117–127. doi: 10.1016/j.freeradbiomed.2007.04.005

Fig. 6.

Fig. 6

BNIP3-mediated mitochondrial dysfunction and cell death. (A) Cells were transfected with empty vector control (EV), BNIP3 or exposed to 400 μM KCN for 24 h. Photomicrographs (400 x) of apoptotic cells stained by TUNEL (dark cells) or Hoechst 33258 stain (bright cells). (B, C) Cells were transiently transfected with empty vector control (EV), BNIP3 or BNIP3ΔTM mutant and then exposed to 400 μM KCN for 24 h in the presence or absence of CsA (1 μM). Then apoptosis was determined (B) and the relative ΔΨm monitored (C). Data represent mean ± SEM of 3–6 separate determinations. *Significantly different from EV-control group; #significantly different from BNIP3-control group; +Signficantly different from BNIP3+KCN group. P<0.05.

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