Figure 2.

Chronic diabetic mice with TB have a similar pattern but increased extent of lung inflammation compared with euglycemic controls. (A) Lung area involved with inflammation of euglycemic and chronic diabetic C57BL/6 mice at different time points after aerosol challenge with Mtb Erdman. Total cross-sectional lung area from all tissues sections was examined and the combined areas of inflammation within these sections were delineated using a Nikon Eclipse E400 microscope (×20 magnification) with Spot Insight v 3.5 software. Percent total area involved with inflammation for two mice per group was calculated as (total area of inflammation/total lung area surveyed) ×100. Open squares: STZ-treated mice with chronic diabetes; closed diamonds: euglycemic control mice. (B, C) Representative hematoxylin and eosin (H&E)-stained lung sections from euglycemic C57BL/6 mouse 12 weeks after infection taken at ×20 and ×200 magnification, respectively. (D, E) Representative H&E-stained lung section from chronic diabetic C57BL/6 mouse 12 weeks after infection at ×20 and ×200 magnification, respectively.