Figure 3.

Chronic diabetic mice have greater leukocyte recruitment to the lungs than euglycemic mice after 16 weeks of TB disease. Isolated lung leukocytes were stained with anti-CD3, -CD4, -CD8, −Gr-1, and -F4/80 mAb for analysis by flow cytometry. (A) The proportion of different leukocyte populations is presented as the mean % surface marker positive cells ± SD (n = 3). Open columns: euglycemic C57BL/6 mice; closed columns: STZ-treated mice with chronic diabetes. (B) The total number of leukocytes of each subset in the lung was determined by multiplying the % surface marker positive cells by the total number of leukocytes isolated from each mouse. Open columns: euglycemic mice; closed columns: STZ-treated mice with chronic diabetes. Data are presented as the mean cell number (×10⁶) ± SD. *P < 0.05, n = 3.