Figure 4.

Lung IFN-γ and inflammatory cytokine expression are increased in acute and chronic diabetic mice late in Mtb infection, while chronic diabetic mice have reduced IFN-γ expression shortly after infection. Lung homogenates were prepared as described in Materials and Methods. (A) Cytokines present in pooled lung lysates prepared from acute diabetic or euglycemic C57BL/6 mice 8 weeks after infection. Closed bars: STZ-treated mice with acute diabetes; open bars: euglycemic control mice; n = 5. (B) Cytokines present in pooled lung lysates prepared from chronic diabetic or euglycemic mice 16 weeks after infection. Closed bars: STZ-treated mice with chronic diabetes; open bars: euglycemic control mice; n = 5. (C) IFN-γ content in lung lysates from individual chronic diabetic C57BL/6 mice 7, 14, 21, and 28 days after infection. Open diamonds: euglycemic control mice; closed diamonds: STZ-treated mice with chronic diabetes; *P < 0.05, n = 5. (D) Cytokines present in pooled lung lysates prepared from chronic diabetic Akita mice or euglycemic C57BL/6 mice 16 weeks after infection. Closed bars: Akita mice with chronic diabetes; open bars: euglycemic control mice; n = 5.