Figure 6.

Antigen-specific and nonspecific IFN-γ responses are reduced in chronic diabetic mice at early time points in TB disease. Chronic diabetic C57BL/6 mice (STZ) and euglycemic controls (CTL) were infected with Mtb Erdman by aerosol. Lung leukocytes harvested 7 and 28 days after infection were cultured on IFN-γ ELIspot plates in the presence of control media, Con A, anti-CD3 mAb, Mtb Erdman CFP, or an ESAT-6 MHC class II peptide. Results are expressed as the mean number of spot-forming cells (SFC) per million cells. *P < 0.05, n = 4.