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. Author manuscript; available in PMC: 2008 Jun 1.
Published in final edited form as: Neuropharmacology. 2007 Mar 24;52(8):1631–1640. doi: 10.1016/j.neuropharm.2007.03.010

Fig.3. Kainate and AMPA enlarge GABAergic terminals.

Fig.3

Summary results on the analysis of eGFP labeled varicosities in axons of cerebellar GABAergic interneurons grown in distinct culture conditions.

A: The percentage increase in the area of the varicosities induced by 0.2, 2, and 20 μM Kainate is compared to the increase obtained in the presence of kainate (K, 20 μM) together with GYKI52466 (GYKI, 25 μM or 50 μM) or MK801 (MK, 10 μM). No change in terminal size was seen with GYKI52466 (GYKI, 50 μM) alone. The area of varicosities in the vehicle group was 0.75 ± 0.18 μm2. * significant to vehicle, p<0.01. + significant to K20, p<0.01.

B: The steep increase in the area of varicosities caused by increasing doses of AMPA (A, 0.2, 2 and 10 μM) is compared to that of NMDA (N, 100 μM). GYKI52466 blocked AMPA but not NMDA-induced increase in the size of the varicosities. In addition it is shown the effect on varicosity enlargement of cyclothiazide (Cyclo, 100 μM) a drug that selectively potentiates the action of flip variants of AMPA receptors. Data derive from at least 30 segments in each experimental group from 6 separate cultures and are expressed as mean ± SD. * significant to vehicle, p<0.01. + significant to A 0.2, p<0.01. × significant to A 2, p<0.01.