Figure 5.

Complementation of the pti1a Mutation with Os Pti1a and Sl Pti1 cDNA.

(A) cDNA of the Os Pti1a or Sl Pti1 genes under control of the 35S promoter was introduced into the Os pti1a homozygous mutant. The photograph was taken 40 d after sowing of transgenic plants (T1 generation) and control plants.

(B) Total RNA was extracted from NB and the pti1a homozygous mutant with the introduced constructs 35S:Os Pti1a or 35S:Sl Pti1 (T1 generation). Ten micrograms of total RNA was hybridized with radiolabeled probes as indicated. The closed arrowhead indicates normal size of Os Pti1a mRNA, and the open arrowhead indicates the size of Os Pti1a with Tos17. Equal loading of RNA samples is shown by the quantity of rRNA.