Figure 3.

BiFC Analysis of the Interaction between 6b and Histone H3 in Leaf Epidermis of N. benthamiana.

nYFP-AK6b was coexpressed with cYFP-H3 ([A] to [C]), nYFP was coexpressed with cYFP-H3 ([D] to [F]), and nYFP-AK6b was coexpressed with cYFP ([G] to [I]). Green fluorescent protein (GFP)-HDT1 and AS2-GFP were included as internal controls. YFP fluorescence due to BiFC is shown ([A], [D], and [G]); GFP fluorescence due to GFP-HDT1 and AS2-GFP is shown ([B], [E], [H], and [J]); and merged views of YFP and GFP fluorescence are shown ([C], [F], and [I]). Insets in (A) to (C) show magnified views of (A) to (C), respectively. The profiles of nuclei are indicated by a dashed line in (C), (F), and (I). Bars = 3 μm in insets in (A) to (C) and 20 μm in all other panels.

(J) The ratio of YFP-positive cells to GFP-positive cells.