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. 1997 Apr 15;94(8):3605–3610. doi: 10.1073/pnas.94.8.3605

Figure 4.

Figure 4

Epitope-tagged nucleolin is found in the LR1–DNA binding complex. PD31 pre-B cells were mock-transfected (−) or transfected with the constructs indicated. (A) Gel mobility-shift analysis of nuclear extracts of PD31 pre-B cells transfected with increasing amounts (1, 2, 4, or 16 μg) of pNtag4, which expresses nucleolin cDNA carrying an N-terminal HA tag. (B) Gel mobility-shift analysis of nuclear extracts from cells transfected with 4 μg pNtag4, which expresses nucleolin cDNA carrying an N-terminal HA tag; or 4 μg pNfor4, which expresses untagged nucleolin cDNA. Reactions were treated with 0.3 or 1 μl of anti-tag mAb 12CA5, as indicated. Control lanes on the right show that the 12CA5 antibody preparation alone did not alter the mobility of the DNA duplex.