Table 1.
Characteristics of the Synechocystis sp PCC 6803 Control Strain A0 and N-Terminal Truncation Mutant A20 Grown at Irradiance of 25 (LL) or 125 (ML) μmol·m−2·s−1
| Strain | Autotrophic Doubling Time (h)a | Oxygen Evolution (μmol O2·mg−1 chlorophyll·h−1)b | TL B Band (°C)c | TL Q Band (°C)d | RCCII(2) (%)e | RCCII(1) (%)e | RC47 (%)e |
|---|---|---|---|---|---|---|---|
| A0 LL | 9.8 ± 0.1 | 640 ± 20 | 28 ± 1 | 10 ± 2 | 48 | 48 | 4 |
| A0 ML | 10.4 ± 0.2 | 820 ± 20 | 28 ± 1 | 9 ± 2 | 48 | 45 | 7 |
| A20 LL | 13.9 ± 0.2 | 560 ± 30 | 28 ± 2 | 9 ± 1 | 36 | 36 | 28 |
| A20 ML | No growth | 430 ± 40 | 28 ± 1 | 10 ± 2 | 18 | 46 | 36 |
Measured on microtitration plates. Values shown are means of 11 measurements ± sd. Initial OD750 nm of the cultures was 0.005.
Light-saturated rate of oxygen evolution measured in the presence of 1 mM p-benzoquinone and 5 mM potassium ferricyanide. Values shown are means of three measurements ± sd. The A20 ML culture for measurement was obtained as described in Methods.
Temperature corresponding to the peak of the thermoluminescence glow curve measured in the range −10 to 70°C after a single-turnover saturating flash given at 5°C in the absence of diuron. Values shown are means of three measurements ± sd.
Temperature corresponding to the peak of the thermoluminescence glow curve measured in the range −10 to 60°C after a single-turnover saturating flash given at −10°C in the presence of 10−5 M diuron. Values shown are means of three measurements ± sd.
Amount obtained by gel densitometry of the CP47 bands from dimeric [RCCII(2)] and monomeric [RCCII(1)] PSII core complexes and core lacking CP43 (RC47) separated by two-dimensional blue-native SDS-PAGE in Figure 5, as quantified by ImageQuant software. Values shown are means of three measurements; sd did not exceed 8%.