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. 2007 Sep;19(9):2776–2792. doi: 10.1105/tpc.107.053678

Figure 5.

Figure 5.

SND1 Binds to a 24-bp Sequence in the MYB46-P6 Fragment.

(A) Diagram of the overlapping DNA oligonucleotides in the MYB46-P6 fragment used in EMSA. The positions of individual oligonucleotides relative to the start codon are shown.

(B) Competition analysis of SND1 binding to MYB46-P6 by the overlapping DNA oligonucleotides shown in (A). Unlabeled double-stranded DNA oligonucleotides (competitors) in 30-fold (+) or 60-fold (++) molar excess relative to the labeled MYB46-P6 probe were used for competition in EMSA. A 24-bp oligonucleotide, MYB46-P6-6, was found to compete with MYB46-P6 for binding to SND1.

(C) Competition analysis of SND1 binding to MYB46-P6 by mutated MYB46-P6-6 oligonucleotides. The wild-type and mutated (M1 to M6) MYB46-P6-6 sequences are shown in the left panel. Dashes in M1 to M6 denote nucleotides that are identical to the wild-type sequence. Unlabeled wild-type and mutated MYB46-P6-6 oligonucleotides in 30-fold molar excess relative to the labeled MYB46-P6 probe were used as competitors in EMSA (right panel).