Figure 1.

Flowering Phenotype and Mapping of the T-DNA Insertion Site in toe1-2D.

(A) Flowering phenotype of toe1-2D. toe1 is a T-DNA insertional knockout mutant (SALK 069677).

(B) T-DNA insertion site in toe1-2D. A single copy of the T-DNA enhancer element is inserted near the TOE1 gene locus in the toe1-2D genome (long arrow). The toe1-1D mutant, which has been characterized by Aukerman and Sakai (2003), is marked by a short arrow. The T-DNA insertional knockout mutant toe1 is marked by an arrowhead.

(C) Growth stage–dependent transcription of TOE1 and other miR172 targets. Transcript levels were examined by RT-PCR–based DNA gel blot analysis. d, days after germination. A tubulin gene (Tub) was included as a control for constitutive expression.

(D) Flowering times of transgenic plants with altered expression of other miR172 target genes. Thirty plants were measured and averaged. Values in the table are means ± se.