Figure 3.

Expression Patterns of Flowering-Time Genes in toe1-2D.

(A) Expression of flowering-time genes in toe1-2D and toe1 examined by RT-PCR–based DNA gel blot hybridization. Ten-day-old plants grown under normal growth conditions (23°C, LD) were harvested at either ZT4 or ZT16 for total RNA extraction. A tubulin gene (Tub) was included as a control for constitutive expression. ZT, Zeitgeber time.

(B) Levels of pre-miR172 and mature miR172 in floral buds and open flowers. A FT-deficient mutant (ft-10) was included in the assays (Yoo et al., 2005). The 5S rRNA served as a loading control.

(C) Flowering times of primary ft-10 transformants overproducing miR172 under LDs (n = 92). The p172a-2 expression construct (Figure 2C) was transformed into ft-10. The arrowhead indicates the range of flowering time of the transgenic plants overproducing miR172 (35S:172a-2), and the arrow marks the range of flowering time of ft-10.

(D) Localized TOE1 expression in rosette leaves compared with that of FT. Promoter sequences of ∼4.5 kb were transcriptionally fused to a β-glucuronidase (GUS)–coding sequence, and the fusion constructs were transformed into Arabidopsis.