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. 2007 Sep;19(9):2719–2735. doi: 10.1105/tpc.106.048769

Figure 4.

Figure 4.

SAW Genes Suppress BP Expression in Leaves.

(A) In situ hybridization showing SAW1, STM and BP expression patterns. (a) and (d) Probed with SAW1 antisense transcript. (b) and (e) Probed with STM antisense transcript. (c) and (f) Probed with BP antisense transcript. (a) to (c) Longitudinal sections; (d) and (e) transverse sections through an inflorescence apex. (f) Longitudinal section through a stage 3 flower. Numbers indicate the floral stages as described by Smyth et al. (1990). sp, sepal; ia, inflorescence apex.

(B) BP is misexpressed in leaves of saw double mutants. (a) Whole mounts of BP:GUS Col (left) and BP:GUS saw1-1 saw2-1 (right) leaves assayed for GUS activity. Arrow points to the BP:GUS expression localized to the hydathodes. (b) and (c) Higher magnification dark-field image of (a). (b) BP:GUS Col and (c) BP:GUS saw1-1 saw2-1. (d) Dark-field image of a whole mount of the ninth leaf of a 4-week-old BP:GUS saw1-1 saw2-1 plant assayed for GUS activity. Arrow points to BP:GUS expression in a secondary serration.