Figure 4.

Dicer-Like Activity Assay in Rice.

(A) A 568-bp dsRNA, flower extract (Flower Ex), or callus extract (Callus Ex) were mixed as shown at top. The siRNAs originating from dsRNA of the GUS gene were detected using the sense strand (top panel) or the antisense strand (bottom panel) as a probe. nt, nucleotides.

(B) The dsRNA was incubated with wild-type or dcl4-1 extract as shown at top. siRNAs were detected by sense strand ³²P-labeled RNA probe. The positions of the produced 21- and 24-nucleotide siRNAs are indicated.