Figure 8.

Expression Pattern of AK120922 Transcript and Its Derived siRNAs in Different Mutants.

(A) Diagrammatic representation of the fold-back structure of AK120922 RNA and the sequence alignment of the fold-back double-stranded sequences. Probes used in the RNA gel blot hybridization are underlined in different colors: red for probe 1, green for probe 2, and blue for probe 3. Probe 1 covers probe 2 and probe 3. Probe 3 represents the region previously identified as miR436. The previously identified siRNA P7-E5 is located within probe 2 and labeled in red.

(B) RNA gel blot analysis of the expression levels of AK120922 in DCL4IR transformants and dcl4-1 using probe 1. nt, nucleotides.

(C) AK120922-related siRNAs in DCL1IR transformants, DCL4IR transformants, and dcl4-1. Probe 1 and probe 2 were ³²P-labeled transcripts, and probe 3 was a specific LNA nucleotide probe.