FIGURE 3.

Effect of IHPK2 mutation on phosphorylation of TAK1 and AKT.a, untransfected (WT), vector-transfected (pCXN2), and mutant-transfected cells were treated with PBS (–) or TNF-α, 15 ng/ml (+) for 1 h. Lysates were subjected to immunoblot with anti-phospho-TAK1 followed by stripping and reprobing with anti-TAK1. Expression of IHPK2 transgene is indicated, and GAPDH served as loading control. Bands were quantitated by densitometry, and -fold induction was calculated (n = 3). b, using the same lysates, similar studies were performed with anti-phospho-AKT and anti-AKT.