Figure 6.

Flavonoid fluorescence localizes differentially following local aglycone applications. CLSM was used to optically section living tt4-1 Arabidopsis roots and to generate cross sections. The indicated aglycones were applied at the root tip (A, C, E, G, and H) or midroot (B, D, and F) of tt4-1 seedlings, incubated for 1 h, DPBA stained, and micrographs collected. The computer-generated cross sections through the brightest zone of fluorescence are directly below the corresponding localization image. Flavonoid fluorescence localized to the pericycle (*), cortex (†), or epidermis (arrows). Scale bars = 50 μm.