Figure 7.

Flavonoids localize intracellularly in the root distal elongation zone after applying aglycones at the root tip. Confocal laser scanning micrographs of a tt4-1 root incubated for 1 h with the indicated compounds applied at the root tip are shown. NaCl at 0.4 m was added to the mounting medium to incipiently plasmolyze the cells. The flavonoid fluorescence shrank with the plasma membrane. The arrows indicate areas where the plasma membrane has receded from the cell wall and trapped the flavonoid fluorescence. The same result occurred for all exogenously applied flavonoid compounds. Scale bars = 50 μm.