Table II.

HPLC analysis of flavonoid intermediates at sites distal to their application

N was applied at the root tip or on cotyledons for 24 h. Seedlings were divided into aerial or root tissue at the root/shoot junction. tt4-1 control seedlings had no detectable flavonoid compounds, even after hydrolysis. The crude extracts or hydrolyzed crude extracts were injected into the HPLC. Statistical analyses compare wild type with the mutants with N applications within the hydrolyzed or unhydrolyzed category using two-tailed Student's t tests with equal or unequal variance depending on F test for variance. Data are the average and se from at least three combined independent experiments.

Seedlings and Conditions	DHK	DHQ	K	Q	DHK	DHQ	K	Q
	Root Tissue				Aerial Tissue
	Unhydrolyzed crude extracts, μg mg−1 fresh weight
Ler	n.d.a	n.d.a	n.d.a	n.d.a	91.8 ± 5.7	152.1 ± 68.3	0.2 ± 0.002	n.d.a
tt4-1 + N root tips	n.d.a	5.9 ± 0.6	n.d.a	n.d.a	204.4 ± 19.4b	287.3 ± 115.9b	2.0 ± 0.19	0.01 ± 0.005
tt4-1 + N leaves	n.d.a	n.d.a	n.d.a	n.d.a	39.1 ± 14.6	128.5 ± 59.5	0.12 ± 0.09	0.002 ± 0.014
	Hydrolyzed crude extracts, μg mg−1 fresh weight
Ler	n.d.a	n.d.a	51.4 ± 24.2	48.2 ± 15.0	29.5 ± 15.5	241.7 ± 69.8	27.8 ± 7.9	11.7 ± 4.0
tt4-1 + N root tips	n.d.a	7.8 ± 0.8	0.3 ± 0.025	n.d.a	23.4 ± 10.5	466.9 ± 27.2b	4.3 ± 0.09b	0.031 ± 0.006b
tt4-1 + N leaves	0.7 ± 0.05	n.d.a	0.23 ± 0.017	0.029 ± 0.005b	13.1 ± 8.1	386.4 ± 27.8	0.78 ± 0.056b	0.06 ± 0.03b

^an.d., Not detected.

^bP < 0.05.