Table III.
Fluorescence movement after using various inhibitors
Inhibitors were supplied to seedlings in the media for 24 h. Seedlings were then transplanted to slides coated with the compounds and supplied with N plus DPBA for 10 min at the root tip. Distances of subsequent flavonoid movement was then tabulated.
| Inhibitor and Concentration | Site of Action and Reference | Fluorescence Movement |
|---|---|---|
| mm | ||
| Control (+N; 100 μm) | – | 9.0 ± 0.4 |
| Bafilomycin A1 (0.3 μm) | Vacuolar H+-ATPase (Scott and Allen, 1999) | 8.2 ± 0.8 |
| Cyclosporine A (0.3 μm) | ABCB transporters (p-glycoproteins; Loyola-Vargas et al., 2007) | 9.9 ± 1.0 |
| Glybenclamide (10 μm) | ABCC transporters (MRP proteins; Forestier et al., 2003) | 7.6 ± 0.4a |
| GSH (250 μm) | Redox (Espunya et al., 2006) | 5.6 ± 0.2a |
| GSSG (250 μm) | Redox (Espunya et al., 2006) | 7.5 ± 0.4a |
| Sodium o-vanadate (100 μm) | ABC transporters (Loyola-Vargas et al., 2007) | 8.0 ± 0.5 |
| NPAb (10 μm) | Auxin transport (Jacobs and Rubery, 1988) | 9.3 ± 0.6 |
| Nigericin (50 nm) | Membrane potentials (Casolo et al., 2003) | 9.9 ± 2.0 |
| Valinomycin (1 μm) | Membrane potentials (Casolo et al., 2003) | 7.3 ± 0.6 |
| Verapamil (100 μm) | ABCB transporters (p-glycoproteins; Loyola-Vargas et al., 2007) | 10.8 ± 2.0 |
a
Statistical analyses compared to control; tested with equal or unequal variance two-tailed Student's t tests depending on F test for variance; P < 0.05, n ≥ 6 replicates.
b
NPA, 1-N-naphthylphthalamic acid.