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. 2007 Oct;145(2):378–388. doi: 10.1104/pp.107.105742

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Copyright © 2007, American Society of Plant Biologists

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Figure 8. — SULTR1;1mycHis and SULTR1;2mycHis proteins are accumulated by short-term sulfate starvation. The transgenic lines and culture conditions were the same as in Figure 7. A, SULTR1;1mycHis proteins in 35S:SULTR1;1mycHis lines (1;1ox-1 and 1;1ox-2). B, SULTR1;2mycHis proteins in 35S:SULTR1;2mycHis lines (1;2ox-11 and 1;2ox-15). One microgram (1;1ox-1) or 5 μg (1;1ox-2, 1;2ox-11, and 1;2ox-15) of crude proteins from root tissues were loaded to each lane of an SDS-polyacrylamide gel, and the accumulation of SULTR1;1mycHis or SULTR1;2mycHis proteins was detected using anti-myc antibody. Gln synthetases GS1 (lower band) and GS2 (upper band) serve as the loading controls.