Figure 2.

Lethal pollen phenotypes in representative transgenic plants harboring RNAi, reporter gene constructs, and empty vectors, respectively. Pollen was isolated from anthers after dehiscence and examined by Alexander staining (Alexander, 1969). Five hundred pollen grains were counted from each T1 plant. A, Wild-type (Columbia) pollen grains. B, Pollen grains from an AGL18∷AGL18 RNAi plant. C, Pollen grains from a 35S∷GFP plant. D, Pollen grains from a transgenic plant harboring the empty vector pGSA1252. Bar = 50 μm. [See online article for color version of this figure.]