Figure 5.

Golgi regeneration after short-term BFA treatment. A, Stacked ER-Golgi hybrid present in wild-type BY-2 cells after 30 min of treatment with 10 μg mL⁻¹ BFA. Intercisternal filaments are visible at different positions throughout the stack. B, Golgi domains in the ER (in brackets) remain recognizable 30 min after BFA washout. C, New Golgi stacks arise although old Golgi domains in the ER are still visible (30 min BFA → 45 min washout). D and E, Massive Golgi stack regeneration after short-term BFA treatment (30 min BFA → 120 min washout; D, confocal image after ARF1 immunostaining; E, chemical-fixed sample.