Table III.

Relative presence of Golgi stacks in tobacco BY-2 cells recovering from BFA treatment (10 μg mL⁻¹) in the presence/absence of inhibitors

Golgi stacks were monitored by ARF1 immunofluorescence, whereby cells were nominally classified as having punctate fluorescence (representing Golgi-bound ARF1) or a diffuse fluorescence (representing cytosolic ARF1). Sample sizes were usually 100 to 200 cells.

Treatment	Percentage of Cells with Labeled Golgi Stacks	Percentage of Cells with Labeled Cytosol
Control	65 ± 5	35 ± 5
50 μm H-89	60 ± 5	40 ± 5
100 μm H-89	55 ± 5	45 ± 5
250 μm H-89	40 ± 5	60 ± 5
500 μm H-89	15 ± 5	85 ± 5
20 μm cytochalasin D	60 ± 5	40 ± 5