Figure 2.

Localizations of CDC48A- or CDC48A^N-D1- and CDC48A^Ndel-YFP. CDC48C-CrFP was used as a nuclear marker (B, E, H, and K). Images were taken at the plane of the nucleus and the merged picture shows the combination of both images together with the chloroplasts in red (C, F, I, and L). CDC48A-YFP localizes to the cytoplasm and the nucleus (compare position of arrowhead in A with image B). The localization at the PM is not visible in this focal plane. CDC48A^N-D1 does localize to the cytoplasm but not to the nucleus (compare the arrowhead in image D with E). CDC48A^Ndel does localize to the cytoplasm and to the nucleus (compare the arrowhead in G with H). The localization of CDC48A^A1A2 (J) is comparable with that of CDC48A (A).