Table III.
Analysis of T1 generation tomatoes transformed with the Atarf8-4 gene
| T1 Generation (Emasculated) | No. of Fruits Developed
|
Average Fruit Size and Weight | Percentage Parthenocarpic Fruit Development | No. of Atarf8-4 Genes | Relative mRNA Expression Ratioa | ||
|---|---|---|---|---|---|---|---|
| Small (<3 cm; <14 g) | Medium (<4 cm; <30 g) | Large (>4 cm; >30 g) | |||||
| ‘Monalbo’ wild type | 73% (n = 64) | 20% (n = 18) | 7% (n = 6) | 2.6 cm ± 0.8 cm 11.06 g ± 10.19 g | 51% | 0 | – |
| ‘Monalbo’ + gAtarf8-4 no. 81 | 65% (n = 21) | 16% (n = 5) | 19% (n = 6) | 2.9 cm ± 0.9 cm 13.54 g ± 10.61 g | 64% | 1 | 1.0 |
| ‘Monalbo’ + gAtarf8-4 no. 6 | 29% (n = 7) | 38% (n = 9) | 33% (n = 8) | 3.3 cm ± 0.7 cm 19.27 g ± 8.80 g | 51% | 3 | 20.1 (±0.8) |
| ‘Monalbo’ + gAtarf8-4 no. 7 | 5% (n = 2) | 12% (n = 5) | 83% (n = 35) | 4.3 cm ± 0.6 cm 34.01 g ± 12.25 g | 72% | 5 | 102.2 (±23.6) |
a
Expression level of Atarf8-4 as determined by real-time PCR from anthesis flowers. There was no expression detectable in the ‘Monalbo’ wild-type flowers. Therefore the expression in line ‘Monalbo’ + gAtarf8-4 no. 81 was set to 1.0 as the reference point.