Figure 1.

Phenotypic and molecular characterization of gh3.5-1D. A, T-DNA insertion in gh3.5-1D. The large arrow indicates the GH3.5 gene. The four cauliflower mosaic virus 35S enhancer elements are indicated with small arrowheads. B, Four-week-old Col-0, heterozygous (gh3.5-1D+/−), homozygous (gh3.5-1D−/−), and representative p35S∷GH3.5 transgenic plant. C, Northern-blot analysis (top) of GH3.5 expression and western-blot detection (bottom) of the GH3.5 protein in Col-0, gh3.5-1D+/−, gh3.5-1D−/−, and p35S∷GH3.5 plants. Rubisco staining was used as a loading control in western blots. The experiments were repeated at least once with similar results.