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Multiple sequence alignment (using EMBL-EBI ClustalW) of three conserved regions of some plant GH32 enzymes. The following sequences were used for the alignment: AtcwINV1, chicory vacuolar invertase, 1-FEH IIa, 1-SST, 1-FFT, and an invertase from yeast. The three crucial catalytic residues are shown in bold. Arrows indicate the residues subjected to mutagenesis. Numbering refers to AtcwINV1.
