Figure 5.

A, Chromatographic pattern of AtcwINV1 wild-type and Asp-239 mutant enzymatic activities. Reaction conditions were as follows: incubation of 50 ng purified enzyme with 10 mm Suc in 50 mm acetate buffer, pH 5.0, during 30 min at 30°C. B, Comparison of specific enzymatic activities [specific (enzymatic) activity in (mol Fru) × (mol enzyme)⁻¹ (s)⁻¹ and (nkat) × (mg protein)⁻¹] of AtcwINV1 wild-type and Asp-239 mutant purified proteins in function of increasing substrate concentrations.