Table I.

Kinetic parameters for the hydrolysis of Suc of purified AtcwINV1 wild-type, D23A mutant, and E203A mutant proteins

Enzyme	Km	kcat	kcat/Km
	mm	s−1	mm−1s−1
Wild type	0.35 ± 0.05	59 ± 4	168.6
D23A	2.4 ± 0.4	10 × 10−2 ± 0.1 × 10−2	42 × 10−3
E203A	2.7 ± 0.3	7 × 10−2 ± 0.5 × 10−2	26 × 10−3