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Kinetic parameters for the hydrolysis of Suc of purified AtcwINV1 wild-type, W20L mutant, W47L mutant, and W82L mutant proteins
| Enzyme | Km | kcat | kcat/Km |
|---|---|---|---|
| mm | s−1 | mm−1s−1 | |
| Wild type | 0.35 ± 0.05 | 59 ± 4 | 168.6 |
| W20L | 72 ± 8 | 89 ± 6 | 1.2 |
| W47L | 212 ± 20 | 175 ± 15 | 0.8 |
| W82L | 43 ± 4 | 39 ± 4 | 0.9 |
