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Kinetic parameters for the hydrolysis of 1-kestose of purified AtcwINV1 wild-type, D239A mutant, and D239F mutant proteins
| Enzyme | Km | kcat | kcat/Km |
|---|---|---|---|
| mm | s−1 | mm−1s−1 | |
| Wild type | 1 ± 0.1 | 21 ± 2 | 21.0 |
| D239A | 0.6 ± 0.03 | 21 ± 3 | 35.0 |
| D239F | 1.2 ± 0.1 | 20 ± 2 | 16.7 |
