Figure 2.
Phenotype of MEm transgenic lines and comparison of senescence parameters with the wild type. A, Images of 4-week-old wild type and transgenic lines grown in long days. The same plants were transferred to prolonged darkness and photographed after 4 d of treatment. B, Chlorophyll content. C, Maximum quantum yield of PSII (FV/FM). D, Total protein content. E, Accumulation of the 54-kD band of the Rubisco large subunit resolved by SDS-PAGE and stained with Coomassie Blue. F, Evans blue accumulation as indicator of cell death was measured spectrophotometrically in the wild type and MEm transformants at different times during extended darkness. Images of Evans blue-stained leaves from the wild type (on the left) and MEm line 4 (on the right) are shown. FW, Fresh weight. Error bars indicate sd (n = 6).