Figure 5.

Deoxynucleotidyl transferase activity of the recombinant AtREV1 protein opposite sites of UV damage. The primer P16, P17A, or P17G was ³²P-labeled at the 5′ end and annealed with template 30T, CPD, or 6-4 (A). The nucleotide sequences around the primer terminus and position of UV damage (T^T for CPD, T = T for 6-4PP) are shown in A. Four hundred nanograms of AtREV1 recombinant protein and the indicated template/primer were incubated with 0.1 mm of each dNTPs under standard reaction conditions with 2 mm magnesium chloride at 30°C for 30 min. The reaction products were resolved in 20% polyacrylamide gels containing 8 m urea and visualized by autoradiography.