Figure 5.
Expression of heat stress-responsive genes in wild type and strs1 and strs2 mutants. A to D, Two-week-old plants were exposed to 40°C for the indicated time periods. Relative transcript levels were determined by real-time PCR according to the 2−ΔΔCT method using UBQ10 as an internal control (Livak and Schmittgen, 2001). Gene expression was normalized to the wild-type unstressed expression level, which was assigned a value of 1. Data represent the average of three independent experiments ± sd.