TLC analysis of wax mixtures from stems and leaves of Arabidopsis wild type, the mah1-1 mutant line, and a transgenic line ectopically overexpressing MAH1. Compound classes are labeled on the left. The standard mixture was composed of tetracosanoic acid (fatty acid), heptacosanol (primary alcohol), nonacosan-11-ol (secondary alcohol), hentriacontan-16-one (ketone), docosyl eicosanoate (ester), and nonacosane (alkane). In the different lanes, wax extracts from wild-type, mutant, or transgenic stems and leaves were separated.