Expression of YUCCA6 cDNA driven by the CaMV 35S promoter recapitulated the yucca6-1D phenotype. A, Mature plants of 3-week-old (top) and 4-week-old (bottom) wild-type, yucca6-1D, and transgenic plants expressing CaMV35S:YUCCA6 (from left to right). B, Phenotypes of transgenic plants depend on expression level of YUCCA6. Both OX5 and OX8 are transgenic plants expressing CaMV35S:YUCCA6. Phenotypes of 3-week-old mature plants (left and middle) and 5-d-old seedlings (right) of OX5 and OX8 are shown. C, The expression levels of YUCCA6 transcripts in wild type, yucca6-1D, OX5, and OX8 were shown in RT-PCR analysis. UBC was used as a control for loading. PCR was performed for 35 cycles with 0.1 μL of cDNA.