Figure 1.

Southern analysis and confirmation of SM1 disruptants. A, Scheme of the gene-deletion strategy. The expected sizes in native (1 kb) and deletion (2.8 kb) events are indicated. The dimensions are not drawn to scale. B, Southern analysis of T. virens wild-type (WT) strain and SM1-deletion transformants (KO25, KO46, and KO70). Autoradiograph of DNA gel blot hybridized with ³²P-dCTP-labeled probe indicated in A. Fifteen micrograms of genomic DNA was digested with HindIII and loaded per lane. Numbers on the right indicate expected size in native and deletion events. Strain 147 contains the native and overexpression bands indicating ectopic integration.