Skip to main content
. 2007 Nov;145(3):712–721. doi: 10.1104/pp.107.103846

Figure 4.

Figure 4.

Characterization of different NEP- or PEP-specific precursor RNAs. Total RNA was prepared from 2-d-old wild-type (lane 1) and 3- to 4-d-old rpoTp (lane 2) or rpoTmp (lane 3) Arabidopsis plantlets and analyzed by primer extension. A, Analyses of RNAs of two exclusively NEP-transcribed genes, e.g. accD and rpoB. The insert on the right shows 5′-RACE of the −538 rpoB transcript with (+) or without (−) TAP treatment of mRNAs. The sequence of the 5′ end and the beginning of the linker is shown below. B, Analyses of RNAs of two exclusively PEP-transcribed genes, rbcL and psbA. C, Analyses of RNAs of two genes, clpP and rrn16S, that are transcribed from nonconsensus-type NEP promoters. The insert on the right shows the PC-initiated transcript after longer exposure. [See online article for color version of this figure.]