Immunoblot analysis of thylakoid proteins separated by BN-PAGE or 2D BN-SDS-PAGE. Thylakoid membranes isolated from wild-type plants. Thylakoids containing 7.5 μg of chl were separated in a BN gel (A), or in the first dimension in a BN gel followed by SDS-PAGE in the second dimension (B). The resolved proteins were immunodetected with anti-D1, anti-CP43, anti-PsbP, anti-PPL1, anti-PPL2, and anti-NdhH antibodies. A.A., Acrylamide.