Table I.
Carbohydrate levels in leaves of three different MP17:GFP transgenic Arabidopsis ecotypes
Leaf samples were harvested 7 h after beginning of the light period. Plants were grown for 5 weeks under SD conditions and samples were collected from fully expanded source leaves. Two samples were taken from each plant and averaged; values represent the mean ± se of 10 (Col-0 and Ler lines) or at least five (C24 lines) independent plants. Asterisks indicate statistical differences between transgenic lines and the respective wild type at *, P < 0.05; **, P < 0.01; and ***, P < 0.001 as determined by the Mann-Whitney U-test.
Plant Line | Glc | Fru | Suc | Starch | Sol Sugars/Starch |
---|---|---|---|---|---|
mmol m−2 | mmol m−2 | mmol m−2 | mmol m−2 | ||
Col-wild type | 0.48 ± 0.06 | 0.92 ± 0.11 | 1.15 ± 0.07 | 11.29 ± 0.46 | 0.23 ± 0.01 |
Col-9 | 0.52 ± 0.06 | 1.87 ± 0.36 | 0.99 ± 0.08 | 10.43 ± 0.56 | 0.32 ± 0.03* |
Col-14 | 1.01 ± 0.15** | 1.64 ± 0.06*** | 1.07 ± 0.07 | 10.88 ± 0.43 | 0.34 ± 0.01*** |
Col-16 | 5.26 ± 0.54*** | 2.11 ± 0.16*** | 2.65 ± 0.24*** | 23.10 ± 2.01*** | 0.44 ± 0.02*** |
C24-wild type | 1.18 ± 0.15 | 1.02 ± 0.18 | 1.00 ± 0.06 | 13.05 ± 0.66 | 0.25 ± 0.03 |
C24-81 | 0.92 ± 0.12 | 0.93 ± 0.12 | 0.78 ± 0.06** | 12.05 ± 0.85 | 0.22 ± 0.01 |
C24-84 | 0.84 ± 0.07 | 1.08 ± 0.09 | 0.79 ± 0.06* | 13.90 ± 0.93 | 0.20 ± 0.01 |
C24-74 | 1.10 ± 0.15 | 1.20 ± 0.10 | 1.09 ± 0.06 | 14.76 ± 0.52 | 0.20 ± 0.01 |
Ler-wild type | 0.82 ± 0.10 | 1.62 ± 0.13 | 1.21 ± 0.04 | 12.04 ± 0.43 | 0.30 ± 0.02 |
Ler-1 | 5.52 ± 0.58*** | 4.72 ± 0.37*** | 2.48 ± 0.15*** | 17.10 ± 1.26** | 0.78 ± 0.09*** |
Ler-12 | 3.49 ± 0.61*** | 3.37 ± 0.29*** | 2.02 ± 0.26** | 13.21 ± 1.43 | 0.76 ± 0.16*** |