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Analysis of the subcellular localization of the CaPO2 protein by transient expression of the CaPO2:smGFP construct in onion epidermal cells. A, Schematic of the smGFP-tagged CaPO2 construct and of a control construct. The smGFP gene was fused to the 3′ region of the CaPO2 gene. B, Transient expression of smGFP or CaPO2:smGFP in onion epidermal cells was detected by confocal laser-scanning microscopy 24 h after biolistic transformation.
