Figure 1.

A, Feulgen staining of B73 roots shows lateral root initiation via emerging primordia. No lateral root primordia are visible 3 d after germination (3 d), whereas primordia were detected at 5 d. Sampling strategy for LCM experiments on 2.5-d-old root: (a) elongation zone; (b) differentiation zone. Approximately 3 mm of the root tip were discarded. Same number of sections of a and b make one biological replicate. Bars indicate 5 mm. B, B73 root cross sections 2.5 d: before and after capturing pericycle, nonpericycle, and nonpericycle central cylinder cells.