. 2007 Nov;145(3):575–588. doi: 10.1104/pp.107.106203

Table IV.

Primary root pericycle proteins isolated from B73 seedlings identified after 2-D electrophoresis and ESI-MS/MS analysis of trypsin-digested proteins matched against NCBI nonredundant protein database entries

AC, Accession no.

Spot No.	Protein [Species] AC^a	Molecular Mass (kD) Predicted/Gel^b	pI Predicted/Gel^c	Sequence Coverage^d	MASCOT Score^e	No. Matched Peptides^f
Signal Transduction^g
15	Guanine nucleotide-binding protein β-subunit protein [O. sativa] NP_001056254	36.4/38	6.06/6.0	10%	128	4
Cellular Organization
5	Actin [Saccharum officinarum] AAU93346	41.7/53	5.24/5.3	12%	171	4
Disease/Defense
18	1-Cys peroxiredoxin antioxidant [Z. mays] ABD24377	25.0/30	6.31/6.2	17%	84	4
19	Pathogenesis-related protein 10 [Z. mays] AAY29574 ^h	16.9/16	5.39/5.3	21%	134	3
Energy
4	ATPase subunit 1 [Z. mays] ABE98710	55.1/62	5.85/5.9	12%	203	6
7	3-Phosphoglycerate kinase [Z. mays] AAO32644	31.6/53	5.01/5.7	26%	465	8
11	Glyceraldehyde-3-P dehydrogenase [Z. mays] Q09054	36.5/43	6.41/6.1	15%	127	5
13	Glyceraldehyde-3-P dehydrogenase [Z. mays] Q09054	36.5/43	6.41/6.3	16%	204	5
12	Glyceraldehyde-3-P dehydrogenase [Z. mays] AAA33466	26.4/42	6.25/6.2	23%	156	5
14	Glyceraldehyde-3-P dehydrogenase [Z. mays] AAA87579	36.4/39	6.61/6.2	18%	255	6
Metabolism
1	Met synthase [Z. mays] AAL33589	84.4/87	5.73/5.6	4%	88	3
2	Phe ammonia-lyase [Z. mays] AAL40137	74.9/75	6.52/6.0	4%	77	3
3	Wheat adenosylhomocysteinase-like protein [O. sativa] AAO72664	53.2/63	5.62/5.6	12%	295	7
6	S-adenosylmethionine synthetase [O. sativa] AAT94053 ⁱ	43.2/55	5.74/5.6	16%	234	6
8	Glu dehydrogenase [Z. mays] AAB51596	44.0/48	5.96/5.9	8%	146	3
9	α-1,4-Glucan-protein synthase [Z. mays] P80607	41.2/45	5.75/5.6	17%	206	7
10	α-1,4-Glucan-protein synthase [Z. mays] P80607	41.2/44	5.75/5.8	9%	69	4
16	Glutathione S-transferase I [Z. mays] P12653	23.8/31	5.44/5.4	15%	250	6
17	Glutathione S-transferase IV [Z. mays] P46420	24.6/30	5.77/5.8	13%	172	4
20	Nucleoside diphosphate kinase [S. officinarum] P93554	16.6/16	6.30/6.2	21%	184	3

Identified proteins obtained via automated algorithm of the MASCOT software (www.matrixscience.com) from the NCBI nonredundant database.

Molecular mass of predicted protein/of protein on gel.

Isoelectric point of predicted protein/of protein on gel.

Percentage of predicted protein sequence covered by matched peptides (cutoff value: 4%).

−10*log(P), where P is the probability that the observed match is a random event. Scores >39 indicate identity or extensive homology (P < 0.05). Protein scores are derived from ion scores as a nonprobabilistic basis for ranking protein hits (cutoff value: >41).

Number of peptides that were identified of a particular protein via ESI-MS/MS (cutoff value: 3).

Classification of the proteins according to Arabidopsis MIPS database (Schoof et al., 2002).

This gene was differentially expressed between pericycle and nonpericycle cells (Tables I and II).

ⁱ

This gene was differentially expressed in a microarray dataset comparing the expression of wild-type versus mutant rum1 pericycle cells (Woll et al., 2005).