Figure 5.

Effect of MeJA on the levels of LUC⁺ reporter mRNA containing the 3′-UTR of AGP31 in transgenic seedlings. A, The construct structure of UBQ10 promoter∷LUC⁺∷AGP31 3′-UTR gene. B, Northern-blot analysis of RNA from transgenic plants carrying a UBQ10 promoter∷LUC⁺∷AGP31 3′-UTR construct. Seven-day-old seedlings of three single-insertion lines were sprayed with 500 μm MeJA and harvested at the given times. The same blot was sequentially hybridized to the following probes: 3′-UTR of AGP31 (to detect LUC⁺ and endogenous AGP31 mRNA), LOX2, and UBQ10.