Table 1.

Dissociation rate constants for yTBP and TBP/ZF at various binding sites

Protein	Binding site	koff, s−1	t½, h
yTBP	GCG/TATA	1.3  ±  0.1 × 10−4	1.5
TBP/ZF	TATA	1.2  ±  0.1 × 10−4	1.6
TBP/ZF	GCG	8.0  ±  1.4 × 10−5	2.4
TBP/ZF	GCG/TATA	4.7  ±  0.8 × 10−7	410
TBP/ZF	TATA/CGC	6.1  ±  0.1 × 10−7	310
TBP/ZF	GCG/AdTATA	3.0  ±  0.3 × 10−7	630
TBP/ZF	AdTATA/CGC	8.6  ±  1.0 × 10−7	220

The dissociation rate constants were determined in three separate experiments. The standard error of the mean is indicated.