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. Author manuscript; available in PMC: 2008 Jun 18.
Published in final edited form as: Brain Res. 2007 Apr 19;1154:40–49. doi: 10.1016/j.brainres.2007.04.026

Figure 1. FGF-2 stimulates activation of Akt and ERK in hippocampal neurons.

Figure 1

Hippocampal neurons were incubated for 5 minutes with the specified concentrations of FGF-2, and lysed. Supernatant was analyzed by immunoblotting with antibody to A) phospho-Akt (p-Akt) and total Akt or B) phospho-ERK (p-ERK) and total ERK. (Bands for both ERK1 (p44) and ERK2 (p42) are visible.) Net intensity of bands was calculated as described in the Methods section, and results are expressed as the means ± S.E.M. of 3 experiments, performed in duplicate. Experimental groups were compared by ANOVA followed by Dunnett’s multiple comparison post tests. **, p<0.01 vs. control. Representative immunoblots are shown.